Skip to main content

LV PACKAGING AND PRODUCER CELL LINES

Our Lenti viral vector stable clonal packaging and producer cell lines offer easy scale up, reduced cost of goods and reduced process complexity. Lenti viral vector packaging and producer cell lines contain the same proven genetic system as the Lenti viral vector transient system, facilitating a smooth transition to this more scalable technology, and retaining all the optimisation benefits developed for the transient system.

"OXGENE's Lenti viral vector packaging and producer cell lines offer a cost-effective solution for large scale cell therapy manufacture"

Lenti viral vector packaging cell lines

OXGENE has successfully developed a suspension-mode, stable lentiviral packaging cell line platform using a proprietary Tet-inducible system. This demonstrates high titre lentiviral production with single plasmid transfection in both shake flask and bioreactor.

Our packaging cell lines, containing inducible VSV-G, Gag-Pol and Rev, have been developed with full traceability from a GMP banked cell line, and provide a simple, flexible platform from which to test multiple transgenes, or variants of a transgene, for further development.

We use a fully defined, serum/animal component free process to generate both our packaging and producer GMP-compliant HEK293 cell lines, which do not contain the SV40 Large-T antigen and remain stable in the absence of antibiotic selection, minimising the regulatory risks in transitioning to clinical use.

Lenti viral vector packaging cell line achieves titres up to 4E7 TU/mL

OXGENE's Lenti viral vector packaging cell line uses only one plasmid and assures reduced inter-batch variability in transfection efficiency compared to the transient system. The Lenti viral vector packaging cell line achieves titres of up to 4E7 TU/mL, compared to the transient system's 8E7 TU/mL.

 

Lenti viral vector producer cell lines

Our packaging cell lines also form the basis of our animal component free custom producer cell lines, derived from a GMP banked master cell line using our automated platform to screen thousands of single cell clones. These permit transfection-free, large-scale production of lentiviral vectors for cell or gene therapies. This reduces the overall cost of goods and process complexity involved in clinical-scale viral vector production, while improving scalability and system reproducibility.

A further advantage of producer cell lines is the added potential for process development to improve viral titres once we've stably integrated your gene of interest, free from the process limitations imposed by transfection. Overall, lentiviral producer cell lines allow the consistent production of large quantities of lentiviral vector at lower cost.

OXGENE’s producer cell lines use our proprietary Lenti viral vector genetic system, which is optimised for translation efficiency, has Tet inducible VSV-G and Gag-Pol expression, and low homology between expression cassettes for an improved safety profile.

We use a fully defined, serum/animal component free process to generate both our packaging and producer GMP-compliant HEK293 cell lines. Cell lines do not contain the SV40 Large-T antigen and remain stable in the absence of antibiotic selection, minimising the regulatory risks in transitioning to clinical use.

Talk to us about Lenti viral vector Packaging
or Producer Cell Lines

 

Are you ready for GMP Manufacture?

WuXi Advanced Therapies is a global Contract Testing, Development
and Manufacturing Organization (CTDMO) dedicated to accelerating
progress and time to market for cell and gene therapies.

×