Cell Line Development Services
OXGENE's team of cell line engineering experts design a strategy and use our optimised workflow and high throughput screening platform to maximise project success across multiple applications, including:
stable transgene integration and expression
increased viral vector or biologic expression
controlled transgene expression or reduced cell toxicity
Clonal Suspension HEK293 Cell lines
Our clonal suspension HEK293 cell lines are validated for high-titre production of recombinant AAV (rAAV), lentiviral and adenoviral vectors. All cell lines, including engineered and media adapted derivatives, offer full traceability and grow in defined, animal component free media. Cell lines do not contain the SV40 large T antigen, which ensures regulatory compliance of derived products for in vivo applications.
Clonal Suspension HEK293TetR Cell Line
Our GMP compliant suspension HEK293TetR cell line is ideal for amplification of cytotoxic adenoviral vectors. We have demonstrated the functionality of this cell line through our partnership with Vaccitech, who are now using this cell line in clinical trials, and through partner CMOs. In all cases, this animal component and antibiotic free cell line outperformed commercial alternatives. It also demonstrated improved scalability and full stability in the absence of antibiotics and allows optimisation of adenoviral production even for transgenes that reduce cell viability.
See the latest data in our HEK293 cell line flyer
We’re also now developing GMP-compliant, suspension mode, stable lentiviral packaging and producer HEK293 cell lines to offer new, industry-leading levels of scalability.
Find out more about XLenti ™ stable cell lines