
We’ve successfully engineered a number of chromosomal translocations using CRISPR/Cas9. Here, we’ve shown RT-PCR (upper panels) and sequencing data (lower panels) confirming gene fusions between (from left to right); Ewing sarcoma breakpoint region 1 (EWSR1) and the ETS transcription factor (FLI1), EMAP like 4 (EML4) and ALK receptor tyrosine kinase (ALK), coiled-coil domain containing 6 (CCDC6) and ROS1, and ETS variant 6 (ETV6) and ABL proto-oncogene 1, non receptor tyrosine kinase (ABL1).