GENE THERAPY SOLUTIONS Plasmids and Cell LinesTransient AAV ProductionTESSA: Scalable AAV Production TRANSIENT AAV PRODUCTION Our industry leading technology platform provides flexible, high titre rAAV production. Reconfigured Rep-Cap and modified Ad5 helper plasmids and GMP banked clonal suspension HEK293 cell line improve AAV yield and quality. "OXGENE's AAV system is superior to off-the-shelf plasmid systems for AAV titres" Andy Topping, Chief Scientific Officer at FUJIFILM Diosynth Biotechnologies Transient AAV Production Our transient expression platform comprises optimised proprietary AAVEX™ plasmids and a GMP-banked clonal suspension HEK293 cell line. We have reconfigured the Rep and Cap genes to place Cap under the control of a strong CMV promoter, while Rep is expressed at a lower level via an EMCV internal ribosome entry site. This results in a higher viral titre, better per cell rAAV productivity and improved full:empty vector ratio. We've also improved adenoviral helper functionality by simplifying the Ad5 helper plasmid, which doesn't contain any adenoviral hexon genes; an added benefit when it comes to regulatory approval. We offer expert cloning and engineering services to customise and optimise our AAVEX™ plasmids for expression of your gene of interest, before process development and manufacture of your rAAV vectors at any grade and scale. Reconfigured Rep-Cap improves viral titre by two to eight fold rAAV production in suspension HEK293 cell line in shake flasks. Using OXGENE’s reconfigured Rep-Cap plasmid (red) improves viral titre between two and eight fold, depending on AAV serotype. Reconfiguration improves particle infectivity rAAV5 made using standard plasmids (left) infects HEK293 cells less efficiently than rAAV5 made using OXGENE's reconfigured RepCap plasmid (right). Reconfiguration improves packaging efficiency Third party evaluation of OXGENE's AAV5 plasmids using four different HEK293 cell lines. Highest and lowest results reported. VG titrated by ddPCR, physical particles by capsid ELISA. From our experts to yours; we offer comprehensive technical support so you can have complete confidence in our technologies. CONTACT US You might be interested inOptimizing Adeno-Associated Virus (AAV) ManufacturingrAAV vector systemAdvances in AAV analyticsOXGENE's AAV Plasmids TESSA™: Scalable AAV Production High-titre, high quality, plasmid-free AAV production. READ NEXT
