Development of a novel mammalian display system for selection of antibodies against membrane protein

Nancy Lopez-Anton, Shalom A. Gurjar, Hena Khalique, Zainab Khalaf, Siobhan Clerkin, Vaughan R. Leydon, Richard Parker-Manuel, Alexander Raeside, Tom Payne, Tim D. Jones, Len Seymour, Ryan Cawood

Abstract

Reliable, specific polyclonal and monoclonal antibodies are important tools in research and medicine. However, the discovery of antibodies against their targets in their native forms is difficult. Here, we present a novel method for discovery of antibodies against membrane proteins in their native configuration in mammalian cells.