A robust pipeline for high throughput CRISPR/Cas-mediated manufacture of engineered knock-out and knock-in cell lines
The capacity to induce genetic modification in mammalian cells using CRISPR/Cas technology has the potential to revolutionise how we understand gene function. However, high throughput application of this technology remains challenging, especially for complex alterations that rely on sporadic activity of the homology directed repair (HDR) pathway. In addition, cell types such as iPSCs are sensitive to these procedures, making it difficult to harness their potential. Here we present an automated, streamlined pipeline for high throughput generation of simple and complex gene modifications in cancer cell lines and iPSCs.
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